•   产品定义

            连锁分析是基于家系群体利用连锁的原理研究相关基因与遗传标记的关系的一种研究方法。根据基因在染色体上呈直线排列、不同基因相互连锁成连锁群的原理，应用被定位的基因与同一染色体上另一基因或遗传标记相连锁的特点进行定位。

            在动植物遗传育种中，通过连锁分析对数量性状位点（Quantitative trait locus,QTL）和质量性状基因进行定位，对于加速育种改良的进程具有重要的意义。

      文库 测序量
    二代测序 350-500bp WGS文库

    亲本个体：≥ 20X

    单个个体：≥ 10X

    测序方案

    参考基因组需求 测序个体数
    是

    亲本：2个

    子代个体：≥ 100个

     

    产品优势

  • 图1 表型相关QTL的LOD值在遗传图谱上的分布

    1. Zou C, Wang P, Xu Y. Bulked sample analysis in genetics, genomics and crop improvement. Plant Biotechnol J. 2016;14(10):1941-1955. doi:10.1111/pbi.12559
    2. Vorobyev A, Gupta Y, Sezin T, et al. Gene-diet interactions associated with complex trait variation in an advanced intercross outbred mouse line. Nat Commun. 2019;10(1):4097. Published 2019 Sep 10. doi:10.1038/s41467-019-11952-w
    3. Wang L, Zhou X, Ren X, et al. A Major and Stable QTL for Bacterial Wilt Resistance on Chromosome B02 Identified Using a High-Density SNP-Based Genetic Linkage Map in Cultivated Peanut Yuanza 9102 Derived Population. Front Genet. 2018;9:652. Published 2018 Dec 18. doi:10.3389/fgene.2018.00652
    4. Mathew LS, Spannagl M, Al-Malki A, et al. A first genetic map of date palm (Phoenix dactylifera) reveals long-range genome structure conservation in the palms. BMC Genomics. 2014;15:285. Published 2014 Apr 15. doi:10.1186/1471-2164-15-285
    5. Tshikunde NM, Mashilo J, Shimelis H, Odindo A. Agronomic and Physiological Traits, and Associated Quantitative Trait Loci (QTL) Affecting Yield Response in Wheat (Triticum aestivum L.): A Review. Front Plant Sci. 2019;10:1428. Published 2019 Nov 5. doi:10.3389/fpls.2019.01428
    6. Wei X, Qiu J, Yong K, et al. A quantitative genomics map of rice provides genetic insights and guides breeding. Nat Genet. 2021;53(2):243-253. doi:10.1038/s41588-020-00769-9
    7. Chen L, Luo J, Jin M, et al. Genome sequencing reveals evidence of adaptive variation in the genus Zea. Nat Genet. 2022;54(11):1736-1745. doi:10.1038/s41588-022-01184-y
    8. Warren WC, Boggs TE, Borowsky R, et al. A chromosome-level genome of Astyanax mexicanus surface fish for comparing population-specific genetic differences contributing to trait evolution. Nat Commun. 2021;12(1):1447. Published 2021 Mar 4. doi:10.1038/s41467-021-21733-z
    9. Bredeson JV, Lyons JB, Oniyinde IO, et al. Chromosome evolution and the genetic basis of agronomically important traits in greater yam. Nat Commun. 2022;13(1):2001. Published 2022 Apr 14. doi:10.1038/s41467-022-29114-w
    10. Li T, Deng G, Su Y, Yang Z, Tang Y, Wang J, Zhang J, Qiu X, Pu X, Yang W, Li J, Liu Z, Zhang H, Liang J, Yu M, Wei Y, Long H. Genetic dissection of quantitative trait loci for grain size and weight by high-resolution genetic mapping in bread wheat (Triticum aestivum L.). Theor Appl Genet. 2022 Jan;135(1):257-271. doi: 10.1007/s00122-021-03964-2. Epub 2021 Oct 13. PMID: 34647130.